• Immunofluorescence analysis of U20S cells using CHD4 antibody (STJ113182) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using CHD4 antibody (STJ113182) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CHD4 antibody (STJ113182) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using CHD4 Rabbit polyclonal antibody (STJ113182) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human oophoroma using CHD4 Rabbit polyclonal antibody (STJ113182) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using CHD4 Rabbit polyclonal antibody (STJ113182) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CHD4 antibody (STJ113182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CHD4 antibody (1520-1690) (STJ113182)

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STJ113182

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHD4 (1520-1690) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHD4
Gene ID: 1108
Uniprot ID: CHD4_HUMAN
Immunogen Region: 1520-1690
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1690 of human CHD4 (NP_001264.2).
Immunogen Sequence: ELAEVEENKKMSQPGSPSPK TPTPSTPGDTQPNTPAPVPP AEDGIKIEENSLKEEESIEG EKEVKSTAPETAIECTQAPA PASEDEKVVVEPPEGEEKVE KAEVKERTEEPMETEPKGAA DVEKVEEKSAIDLTPIVVED KEEKKEEEEKKEVMLQNGET PKDLNDEKQKK
Tissue Specificity Widely expressed.
Function ATP-dependent helicase that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination. Involved in neurogenesis.
Protein Name Chromodomain-Helicase-Dna-Binding Protein 4
Chd-4
Atp-Dependent Helicase Chd4
Mi-2 Autoantigen 218 Kda Protein
Mi2-Beta
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-427389
Reactome: R-HSA-6804758
Reactome: R-HSA-73762
Reactome: R-HSA-8943724
Reactome: R-HSA-9031628
Reactome: R-HSA-9679191
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Associates With Centrosomes In Interphase
Localizes To Sites Of Dna Damage In A Manner Dependent On Zmynd8 And Znf687
Alternative Antibody Names Anti-Chromodomain-Helicase-Dna-Binding Protein 4 antibody
Anti-Chd-4 antibody
Anti-Atp-Dependent Helicase Chd4 antibody
Anti-Mi-2 Autoantigen 218 Kda Protein antibody
Anti-Mi2-Beta antibody
Anti-CHD4 antibody

Information sourced from Uniprot.org

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