Western blot analysis of extracts of various cell lines, using CHCHD2 antibody (STJ119068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHCHD2 antibody (75-145) (STJ119068)

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STJ119068
£129.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHCHD2
Gene ID: 51142
Uniprot ID: CHCH2_HUMAN
Immunogen Region: 75-145
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).
Immunogen Sequence: TLGHAITGGFSGGSNAEPAR PDITYQEPQGTQPAQQQQPC LYEIKQFLECAQNQGDIKLC EGFNEVLKQCR
Function Transcription factor. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen).
Protein Name Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 2
Aging-Associated Gene 10 Protein
Hcv Ns2 Trans-Regulated Protein
Ns2tp
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-9837999
Cellular Localisation Nucleus
Mitochondrion
Mitochondrion Intermembrane Space
Mainly Localized In The Intermembrane Space
Alternative Antibody Names Anti-Coiled-Coil-Helix-Coiled-Coil-Helix Domain-Containing Protein 2 antibody
Anti-Aging-Associated Gene 10 Protein antibody
Anti-Hcv Ns2 Trans-Regulated Protein antibody
Anti-Ns2tp antibody
Anti-CHCHD2 antibody
Anti-C7orf17 antibody
Anti-AAG10 antibody

Information sourced from Uniprot.org