| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CFH (19-323) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CFH |
| Gene ID: |
3075 |
| Uniprot ID: |
CFAH_HUMAN |
| Immunogen Region: |
19-323 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 19-323 of human Complement factor H (NP_000177.2). |
| Immunogen Sequence: |
EDCNELPPRRNTEILTGSWS DQTYPEGTQAIYKCRPGYRS LGNVIMVCRKGEWVALNPLR KCQKRPCGHPGDTPFGTFTL TGGNVFEYGVKAVYTCNEGY QLLGEINYRECDTDGWTNDI PICEVVKCLPVTAPENGKIV SSAMEPDREYHFGQAVRFVC NSGYKIEGDEEMHCSDDGFW SKEKPKCVEISCKSPDVING SPISQKIIYKENERFQYKCN MGYEYSERGDAVCTESGWR |
| Tissue Specificity | Expressed in the retinal pigment epithelium (at protein level). CFH is one of the most abundant complement components in blood where the liver is the major source of CFH protein in vivo. in addition, CFH is secreted by additional cell types including monocytes, fibroblasts, or endothelial cells. |
| Post Translational Modifications | Sulfated on tyrosine residues. |
| Function | Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination. |
| Protein Name | Complement Factor HH Factor 1 |
| Database Links | Reactome: R-HSA-977606 |
| Cellular Localisation | Secreted(Microbial Infection) In The Mosquito MidgutLocalizes To PFalciparum (Nf54 Strain) Macrogamete And Young Zygote Cell Membranes |
| Alternative Antibody Names | Anti-Complement Factor H antibodyAnti-H Factor 1 antibodyAnti-CFH antibodyAnti-HF antibodyAnti-HF1 antibodyAnti-HF2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
