• Immunofluorescence analysis of HeLa cells using CFAP53 Rabbit polyclonal antibody (STJ119034) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CFAP53 Rabbit polyclonal antibody (STJ119034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CFAP53 antibody (1-300) (STJ119034)

SKU:
STJ119034

$151.88 - $708.08
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFAP53
Gene ID: 220136
Uniprot ID: CFA53_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CFAP53 (NP_659457.2).
Immunogen Sequence: MYSQRFGTVQREVKGPTPKV VIVRSKPPKGQGAEHHLERI RRSHQKHNAILASIKSSERD RLKAEWDQHNDCKILDSLVR ARIKDAVQGFIINIEERRNK LRELLALEENEYFTEMQLKK ETIEEKKDRMREKTKLLKEK NEKERQDFVAEKLDQQFRER CEELRVELLSIHQKKVCEER KAQIAFNEELSRQKLVEEQM FSKLWEEDRLAKEKREAQEA RRQKELMENTRLGLNAQIT
Tissue Specificity Expressed in skin fibroblasts (at protein level). Expressed in nasal respiratory epithelial cells (at protein level). Expressed in airway epithelial cells.
Function Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Regulates motility patterns of both 9+0 and 9+2 motile cilia through differential localization and recruitment of axonemal dynein components. Required for centriolar satellite integrity and non-motile cilium assembly. Required for motile cilium formation. Through its role in the beating of primary cilia, involved in the establishment of organ laterality during embryogenesis. Required for sperm flagellum biogenesis and is essential for male fertility.
Protein Name Cilia- And Flagella-Associated Protein 53
Coiled-Coil Domain-Containing Protein 11
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Axoneme
Flagellum Axoneme
Microtubule Organizing Center
Centrosome
Centriole
Centriolar Satellite
Spindle Pole
In Tracheal Cell Cilia
Localizes Prominently To Both Centriolar Satellites And Axonemes
Tightly Associated With Microtubules In Tracheal Cilia
In Embryonic Node Cells
Localizes To The Base Of The Node Cilia At The Centriolar Satellites And
To A Lesser Extent
To The Cilium Axoneme
Localizes To Centriolar Satellites Through G1
S Phase
G2 And Mitosis
Enriched On The Spindle Poles In Mitosis
Relocalizes From The Centriolar Satellite To The Ciliary Transition Zone Upon Ciliogenesis
In Skin Fibroblast Cells
Locates Predominantly To The Centriole With Much Lower Levels Associated With The Actin Cytoskeleton
Localizes To The Sperm Flagellum And Manchette
Alternative Antibody Names Anti-Cilia- And Flagella-Associated Protein 53 antibody
Anti-Coiled-Coil Domain-Containing Protein 11 antibody
Anti-CFAP53 antibody
Anti-CCDC11 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance