• Western blot analysis of extracts of various cell lines, using CETN3 antibody (STJ110410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using CETN3 antibody (STJ110410) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human lung cancer using CETN3 antibody (STJ110410) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of L929 cells using CETN3 antibody (STJ110410) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat brain using CETN3 antibody (STJ110410) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using CETN3 antibody (STJ110410) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody (STJ110410) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using CETN3 antibody (STJ110410) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using CETN3 antibody (A18111) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using CETN3 antibody (STJ110410) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using CETN3 antibody (A18111) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using CETN3 antibody (STJ110410) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using CETN3 antibody (A18111) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CETN3 antibody (STJ110410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CETN3 antibody (1-167) (STJ110410)

SKU:
STJ110410

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CETN3 (1-167) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CETN3
Gene ID: 1070
Uniprot ID: CETN3_HUMAN
Immunogen Region: 1-167
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human CETN3 (NP_004356.2).
Immunogen Sequence: MSLALRSELVVDKTKRKKRR ELSEEQKQEIKDAFELFDTD KDEAIDYHELKVAMRALGFD VKKADVLKILKDYDREATGK ITFEDFNEVVTDWILERDPH EEILKAFKLFDDDDSGKISL RNLRRVARELGENMSDEELR AMIEEFDKDGDGEINQEEFI AIMTGDI
Function Plays a fundamental role in microtubule-organizing center structure and function. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores.
Protein Name Centrin-3
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
Nucleolus
Nucleus Envelope
Nuclear Pore Complex
Centriole
Centrosome Of Interphase And Mitotic Cells
Localizes To Centriole Distal Lumen
Localization At The Nuclear Pore Complex Requires Nup153 And Tpr
Alternative Antibody Names Anti-Centrin-3 antibody
Anti-CETN3 antibody
Anti-CEN3 antibody

Information sourced from Uniprot.org

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