• Western blot analysis of various lysates using CERK Rabbit polyclonal antibody (STJ118334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CERK antibody (C-Term) (STJ118334)

SKU:
STJ118334

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CERK (C-Term) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CERK
Gene ID: 64781
Uniprot ID: CERK1_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human CERK (NP_073603.2).
Immunogen Sequence: CRRSPRGLSPAAHLGDGSSD LILIRKCSRFNFLRFLIRHT NQQDQFDFTFVEVYRVKKFQ FTSKHMEDEDSDLKEGGKKR FGHICSSHPSCCCTVSNSSW NCDGEVLHSPAIEVRVHCQL VRLFARGIEENPKPDSHS
Tissue Specificity High level expression in heart, brain, skeletal muscle, kidney and liver.moderate in peripheral blood leukocytes and thymus.very low in spleen, small intestine, placenta and lung.
Function Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Shows a greater preference for D-erythro isomer of ceramides. Binds phosphoinositides.
Protein Name Ceramide Kinase
Hcerk
Acylsphingosine Kinase
Lipid Kinase 4
Lk4
Database Links Reactome: R-HSA-1660662
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Ceramide Kinase antibody
Anti-Hcerk antibody
Anti-Acylsphingosine Kinase antibody
Anti-Lipid Kinase 4 antibody
Anti-Lk4 antibody
Anti-CERK antibody
Anti-KIAA1646 antibody

Information sourced from Uniprot.org

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