| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CENPF (1730-1890) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CENPF |
| Gene ID: |
1063 |
| Uniprot ID: |
CENPF_HUMAN |
| Immunogen Region: |
1730-1890 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1730-1890 of human CENPF (NP_057427.3). |
| Immunogen Sequence: |
NYEPPGEDKTQGSSECISEL SFSGPNALVPMDFLGNQEDI HNLQLRVKETSNENLRLLHV IEDRDRKVESLLNEMKELDS KLHLQEVQLMTKIEACIELE KIVGELKKENSDLSEKLEYF SCDHQELLQRVETSEGLNSD LEMHADKSSREDIGDNVAKV N |
| Post Translational Modifications | Hyperphosphorylated during mitosis. |
| Function | Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. |
| Protein Name | Centromere Protein FCenp-FAh AntigenKinetochore Protein CenpfMitosin |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-156711Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleus MatrixChromosomeCentromereKinetochoreCytoskeletonSpindleRelocalizes To The Kinetochore/Centromere (Coronal Surface Of The Outer Plate) And The Spindle During MitosisObserved In Nucleus During Interphase But Not In The NucleolusAt Metaphase Becomes Localized To Areas Including Kinetochore And Mitotic Apparatus As Well As CytoplasmBy TelophaseIs Concentrated Within The Intracellular Bridge At Either Side Of The Mid-Body |
| Alternative Antibody Names | Anti-Centromere Protein F antibodyAnti-Cenp-F antibodyAnti-Ah Antigen antibodyAnti-Kinetochore Protein Cenpf antibodyAnti-Mitosin antibodyAnti-CENPF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
