| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CEMIP (180-420) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CEMIP |
| Gene ID: |
57214 |
| Uniprot ID: |
CEMIP_HUMAN |
| Immunogen Region: |
180-420 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 180-420 of human CEMIP (NP_001280227.1). |
| Immunogen Sequence: |
FERSWGHRGVIVHVIDPKSG TVIHSDRFDTYRSKKESERL VQYLNAVPDGRILSVAVNDE GSRNLDDMARKAMTKLGSKH FLHLGFRHPWSFLTVKGNPS SSVEDHIEYHGHRGSAAARV FKLFQTEHGEYFNVSLSSEW VQDVEWTEWFDHDKVSQTKG GEKISDLWKAHPGKICNRPI DIQATTMDGVNLSTEVVYKK GQDYRFACYDRGRACRSYRV RFLCGKPVRPKLTVTIDTN |
| Tissue Specificity | Expressed in dermal and in synovial fibroblasts. Strongly expressed in gastric cancers compared with the paired normal tissues. Strongly expressed in both ductal carcinoma and invasive breast cancer cells compared with benign epithelial cells (at protein level). Strongly expressed in brain, placenta, prostate, breast, lung and testis. Expressed in fibroblasts, epithelial cells and cancer cells. In ear, it is specifically expressed in inner ear. Expressed in cochlea and vestibule tissues. Strongly expressed in gastric cancers compared with the paired normal tissues. Strongly expressed in colon adenocarcinomas compared with normal colonic mucosas. Strongly expressed in breast cancer as compared to normal breast tissue. |
| Post Translational Modifications | N-glycosylated.glycosylation is not necessary for HA-binding. |
| Function | Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing. |
| Protein Name | Cell Migration-Inducing And Hyaluronan-Binding ProteinHyaluronan Binding Protein Involved In Hyaluronan Depolymerization |
| Database Links | Reactome: R-HSA-2142850 |
| Cellular Localisation | NucleusCytoplasmEndoplasmic ReticulumCell MembraneMembraneClathrin-Coated PitSecretedRetained In The Endoplasmic Reticulum (Er) In A Hspa5/Bip-Dependent MannerColocalized With Clathrin Heavy Chain/Cltc In Clathrin-Coated VesiclesStrongly Detected In The Cytoplasm Of Breast Carcinoma CellsWhereas Poorly Detected In Adjacent Normal Epithelial CellsStromal CellsOr Benign Breast TissuesLocalized In The Nucleus And Cytoplasm Of Colon Adenocarcinomas |
| Alternative Antibody Names | Anti-Cell Migration-Inducing And Hyaluronan-Binding Protein antibodyAnti-Hyaluronan Binding Protein Involved In Hyaluronan Depolymerization antibodyAnti-CEMIP antibodyAnti-HYBID antibodyAnti-KIAA1199 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
