| Tissue Specificity | Detected in small intestine, colon and pancreas. |
| Post Translational Modifications | Ubiquitinated, leading to its degradation by the proteasome. Phosphorylation at Ser-60 reduces transactivation capacity. Phosphorylation at Ser-283 reduces transactivation capacity and also increases ubiquitin-dependent proteasome degradation. |
| Function | Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium. Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription. Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription. Binds to and activates transcription of LPH. Activates transcription of CLDN2 and intestinal mucin MUC2. Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA. |
| Protein Name | Homeobox Protein Cdx-2Cdx-3Caudal-Type Homeobox Protein 2 |
| Database Links | Reactome: R-HSA-381771 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Cdx-2 antibodyAnti-Cdx-3 antibodyAnti-Caudal-Type Homeobox Protein 2 antibodyAnti-CDX2 antibodyAnti-CDX3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176591/367442/STJ11103946_1__46971.1713132979.jpg?c=1 "Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176591/367443/STJ11103946_2__65055.1713132979.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176591/367444/STJ11103946_3__52657.1713132980.jpg?c=1 "Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/176591/367442/STJ11103946_1__46971.1713132979.jpg?c=1 "Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit monoclonal antibody (STJ11103946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
