| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CDK7 (211-346) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CDK7 |
| Gene ID: |
1022 |
| Uniprot ID: |
CDK7_HUMAN |
| Immunogen Region: |
211-346 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 211-346 of human CDK7 (NP_001790.1). |
| Immunogen Sequence: |
PFLPGDSDLDQLTRIFETLG TPTEEQWPDMCSLPDYVTFK SFPGIPLHHIFSAAGDDLLD LIQGLFLFNPCARITATQAL KMKYFSNRPGPTPGCQLPRP NCPVETLKEQSNPALAIKRK RTEALEQGGLPKKLIF |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2. |
| Function | Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53.this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition. |
| Protein Name | Cyclin-Dependent Kinase 739 Kda Protein KinaseP39 Mo15Cdk-Activating Kinase 1Cell Division Protein Kinase 7Serine/Threonine-Protein Kinase 1Tfiih Basal Transcription Factor Complex Kinase Subunit |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-6807505Reactome: R-HSA-69202Reactome: R-HSA-69231Reactome: R-HSA-69273Reactome: R-HSA-69656Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075Reactome: R-HSA-8939236 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionColocalizes With Prkci In The Cytoplasm And NucleusTranslocates From The Nucleus To Cytoplasm And Perinuclear Region In Response To Dna-Bound Peptides |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 7 antibodyAnti-39 Kda Protein Kinase antibodyAnti-P39 Mo15 antibodyAnti-Cdk-Activating Kinase 1 antibodyAnti-Cell Division Protein Kinase 7 antibodyAnti-Serine/Threonine-Protein Kinase 1 antibodyAnti-Tfiih Basal Transcription Factor Complex Kinase Subunit antibodyAnti-CDK7 antibodyAnti-CAK antibodyAnti-CAK1 antibodyAnti-CDKN7 antibodyAnti-MO15 antibodyAnti-STK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
