Immunohistochemistry analysis of CDK5RAP1 in paraffin-embedded mouse kidney using CDK5RAP1 Rabbit polyclonal antibody (STJ118289) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CDK5RAP1 antibody (1-350) (STJ118289)

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STJ118289
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK5RAP1
Gene ID: 51654
Uniprot ID: CK5P1_HUMAN
Immunogen Region: 1-350
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CDK5RAP1 (NP_057492.2).
Immunogen Sequence: MHPLQCVLQVQRSLGWGPLA SVSWLSLRMCRAHSSLSSTM CPSPERQEDGARKDFSSRLA AGPTFQHFLKSASAPQEKLS SEVEDPPPYLMMDELLGRQR KVYLETYGCQMNVNDTEIAW SILQKSGYLRTSNLQEADVI LLVTCSIREKAEQTIWNRLH QLKALKTRRPRSRVPLRIGI LGCMAERLKEEILNREKMVD ILAGPDAYRDLPRLLAVAES GQQAANVLLSLDETYADVM
Tissue Specificity Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in neurons of central nervous tissue. Isoform 5: Mainly expressed in brain, placenta and testis. Isoform 6: High expression in placenta and lung.
Function Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp). Essential for efficient and highly accurate protein translation by the ribosome. Specifically inhibits CDK5 activation by CDK5R1. Essential for efficient mitochondrial protein synthesis and respiratory chain.shows pathological consequences in mitochondrial disease.
Protein Name Mitochondrial Trna Methylthiotransferase Cdk5rap1
Cdk5 Activator-Binding Protein C42
Cdk5 Regulatory Subunit-Associated Protein 1
Mt-Trna-2-Methylthio-N6-Dimethylallyladenosine Synthase
Mt-Trna-N6-(Dimethylallyladenosine(37 Methylthiotransferase
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Mitochondrial Trna Methylthiotransferase Cdk5rap1 antibody
Anti-Cdk5 Activator-Binding Protein C42 antibody
Anti-Cdk5 Regulatory Subunit-Associated Protein 1 antibody
Anti-Mt-Trna-2-Methylthio-N6-Dimethylallyladenosine Synthase antibody
Anti-Mt-Trna-N6-(Dimethylallyladenosine(37 Methylthiotransferase antibody
Anti-CDK5RAP1 antibody
Anti-C20orf34 antibody
Anti-CGI-05 antibody
Anti-HSPC167 antibody

Information sourced from Uniprot.org

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