• Immunofluorescence analysis of L929 cells using CDH11 Rabbit polyclonal antibody (STJ114069) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CDH11 Rabbit polyclonal antibody (STJ114069) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-CDH11 antibody (120-796) (STJ114069)

SKU:
STJ114069

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDH11 (120-796) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH11
Gene ID: 1009
Uniprot ID: CAD11_HUMAN
Immunogen Region: 120-796
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-796 of human CDH11 (NP_001788.2).
Immunogen Sequence: ERAQYTLMAQAVDRDTNRPL EPPSEFIVKVQDINDNPPEF LHETYHANVPERSNVGTSVI QVTASDADDPTYGNSAKLVY SILEGQPYFSVEAQTGIIRT ALPNMDREAKEEYHVVIQAK DMGGHMGGLSGTTKVTITLT DVNDNPPKFPQSVYQMSVSE AAVPGEEVGRVKAKDPDIGE NGLVTYNIVDGDGMESFEIT TDYETQEGVIKLKKPVDFET KRAYSLKVEAANVHIDPKF
Tissue Specificity Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts. In the embryo from 67 to 72 days of gestation, detected at high levels in facial mesenchyme including the central palatal mesenchyme, dental mesenchyme, the eye and optic muscle, and the tongue (at protein level).
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. Required for proper focal adhesion assembly. Involved in the regulation of cell migration.
Protein Name Cadherin-11
Osf-4
Osteoblast Cadherin
Ob-Cadherin
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-9759811 P55287-2
Reactome: R-HSA-9762292 P55287-2
Reactome: R-HSA-9833576
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cadherin-11 antibody
Anti-Osf-4 antibody
Anti-Osteoblast Cadherin antibody
Anti-Ob-Cadherin antibody
Anti-CDH11 antibody

Information sourced from Uniprot.org

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