• Immunohistochemistry analysis of paraffin-embedded rat kidney using CDCA8 antibody (STJ114468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using CDCA8 antibody (STJ114468) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of Mouse testis, using CDCA8 antibody (STJ114468) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CDCA8 antibody (1-280) (STJ114468)

SKU:
STJ114468

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDCA8 (1-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDCA8
Gene ID: 55143
Uniprot ID: BOREA_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CDCA8 (NP_060571.1).
Immunogen Sequence: MAPRKGSSRVAKTNSLRRRK LASFLKDFDREVEIRIKQIE SDRQNLLKEVDNLYNIEILR LPKALREMNWLDYFALGGNK QALEEAATADLDITEINKLT AEAIQTPLKSAKTRKVIQVD EMIVEEEEEEENERKNLQTA RVKRCPPSKKRTQSIQGKGK GKRSSRANTVTPAVGRLEVS MVKPTPGLTPRFDSRVFKTP GLRTPAAGERIYNISGNGSP LADSKEIFLTVPVGGGESL
Post Translational Modifications Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA. Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
Function Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Protein Name Borealin
Cell Division Cycle-Associated Protein 8
Dasra-B
Hdasra-B
Pluripotent Embryonic Stem Cell-Related Gene 3 Protein
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-4615885
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Cytoskeleton
Spindle
Chromosome
Centromere
Localizes On Chromosome Arms And Inner Centromeres From Prophase Through Metaphase And Then Transferring To The Spindle Midzone And Midbody From Anaphase Through Cytokinesis
Colocalizes With Senp3 In The Nucleolus In Interphase Cells
Alternative Antibody Names Anti-Borealin antibody
Anti-Cell Division Cycle-Associated Protein 8 antibody
Anti-Dasra-B antibody
Anti-Hdasra-B antibody
Anti-Pluripotent Embryonic Stem Cell-Related Gene 3 Protein antibody
Anti-CDCA8 antibody
Anti-PESCRG3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance