Anti-CDCA8 antibody (1-280) (STJ114468)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CDCA8 (1-280) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDCA8 |
| Gene ID | 55143 |
| Uniprot ID | BOREA_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MAPRKGSSRVAKTNSLRRRK LASFLKDFDREVEIRIKQIE SDRQNLLKEVDNLYNIEILR LPKALREMNWLDYFALGGNK QALEEAATADLDITEINKLT AEAIQTPLKSAKTRKVIQVD EMIVEEEEEEENERKNLQTA RVKRCPPSKKRTQSIQGKGK GKRSSRANTVTPAVGRLEVS MVKPTPGLTPRFDSRVFKTP GLRTPAAGERIYNISGNGSP LADSKEIFLTVPVGGGESL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CDCA8 (NP_060571.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA. Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. |
| Function | Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. |
| Protein Name | BorealinCell Division Cycle-Associated Protein 8Dasra-BHdasra-BPluripotent Embryonic Stem Cell-Related Gene 3 Protein |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-4615885Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusNucleolusCytoplasmCytoskeletonSpindleChromosomeCentromereLocalizes On Chromosome Arms And Inner Centromeres From Prophase Through Metaphase And Then Transferring To The Spindle Midzone And Midbody From Anaphase Through CytokinesisColocalizes With Senp3 In The Nucleolus In Interphase Cells |
| Alternative Antibody Names | Anti-Borealin antibodyAnti-Cell Division Cycle-Associated Protein 8 antibodyAnti-Dasra-B antibodyAnti-Hdasra-B antibodyAnti-Pluripotent Embryonic Stem Cell-Related Gene 3 Protein antibodyAnti-CDCA8 antibodyAnti-PESCRG3 antibody |
Information sourced from Uniprot.org