| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Cell division cycle-associated protein 2 (511-560 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDCA2 |
| Gene ID: | 157313 |
| Uniprot ID: | CDCA2_HUMAN |
| Immunogen Region: | 511-560 aa |
| Specificity: | Repo-Man Polyclonal Antibody detects endogenous levels of Repo-Man protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human CDCA2 at the amino acid range 511-560 |
| Post Translational Modifications | Phosphorylated by CDK1. May regulate its subcellular location. |
| Function | Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates. |
| Protein Name | Cell Division Cycle-Associated Protein 2Recruits Pp1 Onto Mitotic Chromatin At Anaphase ProteinRepo-Man |
| Cellular Localisation | NucleusExcluded From The NucleolusPresent In Nucleoplasm Throughout The G1S And G2 Stages Of The Cell CycleDuring M PhaseIt Becomes Diffuse Throughout The Cell As The Nuclear Membrane Breaks DownAnd Faintly Accumulates Later On Metaphase ChromatinAs The Cell Progresses To AnaphaseIt Accumulates On Chromatin |
| Alternative Antibody Names | Anti-Cell Division Cycle-Associated Protein 2 antibodyAnti-Recruits Pp1 Onto Mitotic Chromatin At Anaphase Protein antibodyAnti-Repo-Man antibodyAnti-CDCA2 antibody |
Information sourced from Uniprot.org