Anti-CDC5L antibody (721-770 aa) (STJ92180)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CDC5L at the amino acid range 721-770
General Information
| Short Description | Rabbit polyclonal anti-Cell division cycle 5-like protein (721-770 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDC5L |
| Gene ID | 988 |
| Uniprot ID | CDC5L_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CDC5L at the amino acid range 721-770 |
| Immunogen Region | 721-770 aa |
| Specificity | Cdc5L Polyclonal Antibody detects endogenous levels of Cdc5L protein. |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8. |
| Function | DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). |
| Protein Name | Cell Division Cycle 5-Like ProteinCdc5-Like ProteinPombe Cdc5-Related Protein |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusNucleus SpeckleCytoplasmMay Shuttle Between Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Cell Division Cycle 5-Like Protein antibodyAnti-Cdc5-Like Protein antibodyAnti-Pombe Cdc5-Related Protein antibodyAnti-CDC5L antibodyAnti-KIAA0432 antibodyAnti-PCDC5RP antibody |
Information sourced from Uniprot.org