| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Cell division cycle 5-like protein (721-770 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDC5L |
| Gene ID: | 988 |
| Uniprot ID: | CDC5L_HUMAN |
| Immunogen Region: | 721-770 aa |
| Specificity: | Cdc5L Polyclonal Antibody detects endogenous levels of Cdc5L protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human CDC5L at the amino acid range 721-770 |
| Post Translational Modifications | Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8. |
| Function | DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). |
| Protein Name | Cell Division Cycle 5-Like Protein Cdc5-Like Protein Pombe Cdc5-Related Protein |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus Nucleus Speckle Cytoplasm May Shuttle Between Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Cell Division Cycle 5-Like Protein antibody Anti-Cdc5-Like Protein antibody Anti-Pombe Cdc5-Related Protein antibody Anti-CDC5L antibody Anti-KIAA0432 antibody Anti-PCDC5RP antibody |
Information sourced from Uniprot.org