| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDC42EP3 |
| Gene ID: | 10602 |
| Uniprot ID: | BORG2_HUMAN |
| Immunogen Region: | 1-254 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human CDC42EP3 (NP_006440.2). |
| Immunogen Sequence: | MPAKTPIYLKAANNKKGKKF KLRDILSPDMISPPLGDFRH TIHIGKEGQHDVFGDISFLQ GNYELLPGNQEKAHLGQFPG HNEFFRANSTSDSVFTETPS PVLKNAISLPTIGGSQALML PLLSPVTFNSKQESFGPAKL PRLSCEPVMEEKAQEKSSLL ENGTVHQGDTSWGSSGSASQ SSQGRDSHSSSLSEQYPDWP AEDMFDHPTPCELIKGKTKS EESLSDLTGSLLSLQLDLG |
| Tissue Specificity | Highly expressed in the heart and weakly in the brain. |
| Function | Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. |
| Protein Name | Cdc42 Effector Protein 3Binder Of Rho Gtpases 2Mse55-Related Cdc42-Binding Protein |
| Database Links | Reactome: R-HSA-5687128Reactome: R-HSA-9013148Reactome: R-HSA-9013406 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinCytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Cdc42 Effector Protein 3 antibodyAnti-Binder Of Rho Gtpases 2 antibodyAnti-Mse55-Related Cdc42-Binding Protein antibodyAnti-CDC42EP3 antibodyAnti-BORG2 antibodyAnti-CEP3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98779/382828/STJ119994_1__38128.1713151329.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
