| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000IHC-P:1:50-1:100IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CDC20 |
| Gene ID: |
991 |
| Uniprot ID: |
CDC20_HUMAN |
| Immunogen Region: |
1-100 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CDC20 (NP_001246.2?). |
| Immunogen Sequence: |
MAQFAFESDLHSLLQLDAPI PNAPPARWQRKAKEAAGPAP SPMRAANRSHSAGRTPGRTP GKSSSKVQTTPSKPGGDRYI PHRSAAQMEVASFLLSKENQ |
| Post Translational Modifications | Acetylated. Deacetylated at Lys-66 by SIRT2.deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C). Phosphorylated during mitosis. Phosphorylated by BUB1 at Ser-41.Ser-72.Ser-92.Ser-153.Thr-157 and Ser-161. Phosphorylated by NEK2. Dephosphorylated by CTDP1. Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex. Ubiquitinated by UBR5 when not assembled in a multiprotein complex, leading to its degradation: UBR5 recognizes and binds a degron that is not accessible when CDC20 is part of a complex. |
| Function | Involved in the metaphase/anaphase transition of cell cycle. Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and confers substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41. |
| Protein Name | Cell Division Cycle Protein 20 HomologP55cdc |
| Database Links | Reactome: R-HSA-141405Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174048Reactome: R-HSA-174113Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176417Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-5689880Reactome: R-HSA-68877Reactome: R-HSA-9648025Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreSpindle Pole |
| Alternative Antibody Names | Anti-Cell Division Cycle Protein 20 Homolog antibodyAnti-P55cdc antibodyAnti-CDC20 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
