| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CD98 (206-630) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SLC3A2 |
| Gene ID: |
6520 |
| Uniprot ID: |
4F2_HUMAN |
| Immunogen Region: |
206-630 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 206-630 of human SLC3A2/CD98hc (NP_002385.3). |
| Immunogen Sequence: |
RAPRCRELPAQKWWHTGALY RIGDLQAFQGHGAGNLAGLK GRLDYLSSLKVKGLVLGPIH KNQKDDVAQTDLLQIDPNFG SKEDFDSLLQSAKKKSIRVI LDLTPNYRGENSWFSTQVDT VATKVKDALEFWLQAGVDGF QVRDIENLKDASSFLAEWQN ITKGFSEDRLLIAGTNSSDL QQILSLLESNKDLLLTSSYL SDSGSTGEHTKSLVTQYLNA TGNRWCSWSLSQARLLTSF |
| Tissue Specificity | Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1. |
| Post Translational Modifications | N-glycosylated.N-glycosylation is crucial for trafficking and stability of SLC3A2 to the plasma membrane. Phosphorylation on Ser-406.Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions. |
| Function | Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters. Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids. Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane. SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA. SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression. (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis. (Microbial infection) Acts as a receptor for malaria parasite Plasmodium vivax (Thai isolate) in immature red blood cells. |
| Protein Name | Amino Acid Transporter Heavy Chain Slc3a24f2 Cell-Surface Antigen Heavy Chain4f2hc4f2 Heavy Chain AntigenLymphocyte Activation Antigen 4f2 Large SubunitSolute Carrier Family 3 Member 2Cd Antigen Cd98 |
| Database Links | Reactome: R-HSA-210991Reactome: R-HSA-352230Reactome: R-HSA-5660862Reactome: R-HSA-71240 |
| Cellular Localisation | Apical Cell MembraneCell MembraneSingle-Pass Type Ii Membrane ProteinCell JunctionLysosome MembraneMelanosomeBasolateral Cell MembraneLocalized At The Plasma Membrane When Associated With Slc7a5/Lat1 Or Slc7a8/Lat2Localized To The Apical Membrane Of Placental Syncytiotrophoblastic CellsRecruited To Lysosomes By Laptm4bIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocated Selectively At Cell-Cell Adhesion SitesColocalized With Slc7a8/Lat2 At The Basolateral Membrane Of Kidney Proximal Tubules And Small Intestine EpitheliaExpressed In Both Luminal And Abluminal Membranes Of Brain Capillary Endothelial Cells |
| Alternative Antibody Names | Anti-Amino Acid Transporter Heavy Chain Slc3a2 antibodyAnti-4f2 Cell-Surface Antigen Heavy Chain antibodyAnti-4f2hc antibodyAnti-4f2 Heavy Chain Antigen antibodyAnti-Lymphocyte Activation Antigen 4f2 Large Subunit antibodyAnti-Solute Carrier Family 3 Member 2 antibodyAnti-Cd Antigen Cd98 antibodyAnti-SLC3A2 antibodyAnti-MDU1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
