• Western blot analysis of extracts of various cell lines, using CD72 antibody (STJ111971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of Daudi, using CD72 antibody (STJ111971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD72 antibody (120-359) (STJ111971)

SKU:
STJ111971

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD72
Gene ID: 971
Uniprot ID: CD72_HUMAN
Immunogen Region: 120-359
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 120-359 of human CD72 (NP_001773.1).
Immunogen Sequence: QVSQQLQQTNRVLEVTNSSL RQQLRLKITQLGQSAEDLQG SRRELAQSQEALQVEQRAHQ AAEGQLQACQADRQKTKETL QSEEQQRRALEQKLSNMENR LKPFFTCGSADTCCPSGWIM HQKSCFYISLTSKNWQESQK QCETLSSKLATFSEIYPQSH SYYFLNSLLPNGGSGNSYWT GLSSNKDWKLTDDTQRTRTY AQSSKCNKVHKTWSWWTLES ESCRSSLPYICEMTAFRFP
Tissue Specificity Pre-B-cells and B-cells but not terminally differentiated plasma cells.
Post Translational Modifications Phosphorylated upon engagement of the B-cell receptor, probably by LYN or SYK. Phosphorylation at Tyr-7 is important for interaction with PTPN6/SHP-1.
Function Co-receptor of B cell receptor (BCR) that plays both positive and negative roles on B-cell functions. Recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand, and coligation of CD72 and BCR inhibits BCR signaling. Mechanistically, ligand binding leads to the recruitment of PTPN6/SHP-1 to the BCR complex which is inhibitory to BCR signaling. Acts also as a ligand for CD5 and thereby plays a critical role in maintaining regulatory T and B-cell homeostasis.
Protein Name B-Cell Differentiation Antigen Cd72
Lyb-2
Cd Antigen Cd72
Database Links Reactome: R-HSA-416700
Cellular Localisation Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-B-Cell Differentiation Antigen Cd72 antibody
Anti-Lyb-2 antibody
Anti-Cd Antigen Cd72 antibody
Anti-CD72 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance