| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD59 |
| Gene ID: | 966 |
| Uniprot ID: | CD59_HUMAN |
| Immunogen Region: | 26-128 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-128 of human CD59 (NP_001120695.1). |
| Immunogen Sequence: | LQCYNCPNPTADCKTAVNCS SDFDACLITKAGLQVYNKCW KFEHCNFNDVTTRLRENELT YYCCKKDLCNFNEQLENGGT SLSEKTVLLLVTPFLAAAWS LHP |
| Post Translational Modifications | N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. |
| Function | Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. |
| Protein Name | Cd59 Glycoprotein 1f5 Antigen 20 Kda Homologous Restriction Factor Hrf-20 Hrf20 Mac-Inhibitory Protein Mac-Ip Mem43 Antigen Membrane Attack Complex Inhibition Factor Macif Membrane Inhibitor Of Reactive Lysis Mirl Protectin Cd Antigen Cd59 |
| Database Links | Reactome: R-HSA-204005 Reactome: R-HSA-5694530 Reactome: R-HSA-6798695 Reactome: R-HSA-6807878 Reactome: R-HSA-977606 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor Secreted Soluble Form Found In A Number Of Tissues |
| Alternative Antibody Names | Anti-Cd59 Glycoprotein antibody Anti-1f5 Antigen antibody Anti-20 Kda Homologous Restriction Factor antibody Anti-Hrf-20 antibody Anti-Hrf20 antibody Anti-Mac-Inhibitory Protein antibody Anti-Mac-Ip antibody Anti-Mem43 Antigen antibody Anti-Membrane Attack Complex Inhibition Factor antibody Anti-Macif antibody Anti-Membrane Inhibitor Of Reactive Lysis antibody Anti-Mirl antibody Anti-Protectin antibody Anti-Cd Antigen Cd59 antibody Anti-CD59 antibody Anti-MIC11 antibody Anti-MIN1 antibody Anti-MIN2 antibody Anti-MIN3 antibody Anti-MSK21 antibody |
Information sourced from Uniprot.org