• Western blot analysis of Mouse spleen, using CD53 Rabbit polyclonal antibody (STJ11105394) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD53 antibody (107-181) (STJ11105394)

SKU:
STJ11105394

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD53 (107-181) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Cd53
Gene ID: 12508
Uniprot ID: CD53_MOUSE
Immunogen Region: 107-181
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 107-181 of mouse CD53 (NP_031677.1).
Immunogen Sequence: EQKLNTLVAEGLNDSIQHYH SDNSTMKAWDFIQTQLQCCG VNGSSDWTSGPPSSCPSGAD VQGCYNKAKSWFHSN
Function Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells.
Protein Name Leukocyte Surface Antigen Cd53
Cell Surface Glycoprotein Cd53
Cd Antigen Cd53
Database Links Reactome: R-MMU-6798695
Cellular Localisation Cell Membrane
Cell Junction
Membrane
Multi-Pass Membrane Protein
Concentrates In Localized Microdomains Along The Plasma Membrane At The Contact Sites Between Cells Of Fused Myotubes
Alternative Antibody Names Anti-Leukocyte Surface Antigen Cd53 antibody
Anti-Cell Surface Glycoprotein Cd53 antibody
Anti-Cd Antigen Cd53 antibody
Anti-Cd53 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance