| Short Description : | Rabbit polyclonal CD53 (107-181) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Cd53 |
| Gene ID: | 12508 |
| Uniprot ID: | CD53_MOUSE |
| Immunogen Region: | 107-181 |
| Immunogen Sequence: | EQKLNTLVAEGLNDSIQHYH SDNSTMKAWDFIQTQLQCCG VNGSSDWTSGPPSSCPSGAD VQGCYNKAKSWFHSN |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 107-181 of mouse CD53 (NP_031677.1). |
| Function | Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Plays a role in the activation of monocytes and B-cells. Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling. Also promotes, in B-cells, the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates. Plays an essential role in lymphocyte homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression. Also mediates metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment. Protects hematopoietic stem cell function in response to stress by facilitating DREAM complex activity through association with p130/RBL2 and its phosphatase PP2A. |
| Protein Name | Leukocyte Surface Antigen Cd53Cell Surface Glycoprotein Cd53Cd Antigen Cd53 |
| Database Links | Reactome: R-MMU-6798695 |
| Cellular Localisation | Cell MembraneCell JunctionMembraneMulti-Pass Membrane ProteinConcentrates In Localized Microdomains Along The Plasma Membrane At The Contact Sites Between Cells Of Fused Myotubes |
| Alternative Antibody Names | Anti-Leukocyte Surface Antigen Cd53 antibodyAnti-Cell Surface Glycoprotein Cd53 antibodyAnti-Cd Antigen Cd53 antibodyAnti-Cd53 antibody |
Information sourced from Uniprot.org
