Anti-CD248 antibody (18-150) (STJ114576)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CD248 (18-150) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD248 |
| Gene ID | 57124 |
| Uniprot ID | CD248_HUMAN |
| Immunogen Region | 18-150 |
| Immunogen Sequence | QDPWAAEPRAACGPSSCYAL FPRRRTFLEAWRACRELGGD LATPRTPEEAQRVDSLVGAG PASRLLWIGLQRQARQCQLQ RPLRGFTWTTGDQDTAFTNW AQPASGGPCPAQRCVALEAS GEHRWLEGSCTLA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 18-150 of human CD248 (NP_065137.1). |
Additional Info
| Tissue Specificity | Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. Strongly expressed in pericytes. Expressed on stromal cells and cells with lymphoid morphology such a T-cells. |
| Post Translational Modifications | O-glycosylated with sialylated oligosaccharides. May be N-glycosylated. |
| Function | Cell surface glycoprotein involved in various biological processes including angiogenesis, immune response modulation, and tissue remodeling and repair. Participates in pericyte proliferation through positive modulation of the PDGF receptor signaling pathway. Acts as a scaffold for factor X, triggering allosteric changes and the spatial re-alignment of factor X with the TF-factor VIIa complex, thereby enhancing coagulation activation. Modulates the insulin signaling pathway by interacting with insulin receptor/INSR and by diminishing its capacity to be autophosphorylated in response to insulin. Also regulates LPS-induced inflammatory response in macrophages by favoring the production of proinflammatory cytokines. In human, negatively regulates T-cell proliferation compared with stromal cells where it increases proliferation. |
| Protein Name | EndosialinTumor Endothelial Marker 1Cd Antigen Cd248 |
| Database Links | |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Endosialin antibodyAnti-Tumor Endothelial Marker 1 antibodyAnti-Cd Antigen Cd248 antibodyAnti-CD248 antibodyAnti-CD164L1 antibodyAnti-TEM1 antibody |
Information sourced from Uniprot.org