• Western blot analysis of various lysates, using CD2 antibody (STJ117816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD2 rabbit polyclonal antibody (STJ117816) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD2 antibody (235-351) (STJ117816)

SKU:
STJ117816

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD2 (235-351) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD2
Gene ID: 914
Uniprot ID: CD2_HUMAN
Immunogen Region: 235-351
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 235-351 of human CD2 (NP_001758.2).
Immunogen Sequence: TKRKKQRSRRNDEELETRAH RVATEERGRKPHQIPASTPQ NPATSQHPPPPPGHRSQAPS HRPPPPGHRVQHQPQKRPPA PSGTQVHQQKGPPLPRPRVQ PKPPHGAAENSLSPSSN
Tissue Specificity Expressed in natural killer cells (at protein level).
Function CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Protein Name T-Cell Surface Antigen Cd2
Erythrocyte Receptor
Lfa-2
Lfa-3 Receptor
Rosette Receptor
T-Cell Surface Antigen T11/Leu-5
Cd Antigen Cd2
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-T-Cell Surface Antigen Cd2 antibody
Anti-Erythrocyte Receptor antibody
Anti-Lfa-2 antibody
Anti-Lfa-3 Receptor antibody
Anti-Rosette Receptor antibody
Anti-T-Cell Surface Antigen T11/Leu-5 antibody
Anti-Cd Antigen Cd2 antibody
Anti-CD2 antibody
Anti-SRBC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance