• Western blot analysis of 1) Jurkat, 2) K562, diluted at 1:2000.
  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, CD16 monoclonal antibody (Q32) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CD16 antibody [Q32] (STJ96992)

SKU:
STJ96992

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Low affinity immunoglobulin gamma Fc region receptor III-A and Low affinity immunoglobulin gamma Fc region receptor III-B is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applica
Clonality: Monoclonal
Clone ID: Q32
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000
IHC 1:50-300
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FCGR3B
FCGR3A
Gene ID: 2215
2214
Uniprot ID: FCG3B_HUMAN
FCG3A_HUMAN
Specificity: The antibody detects endogenous CD16 protein.
Immunogen: Synthetic Peptide of CD16
Post Translational Modifications Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. The soluble form is produced by a proteolytic cleavage.
Function Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Protein Name Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii-A
Cd16a Antigen
Fc-Gamma Riii-Alpha
Fc-Gamma Riii
Fc-Gamma Riiia
Fcriii
Fcriiia
Fcr-10
Igg Fc Receptor Iii-2
Cd Antigen Cd16a
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-2029481
Reactome: R-HSA-2029482
Reactome: R-HSA-2029485
Reactome: R-HSA-9664323
Reactome: R-HSA-9664422
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Secreted
Exists Also As A Soluble Receptor
Alternative Antibody Names Anti-Low Affinity Immunoglobulin Gamma Fc Region Receptor Iii-A antibody
Anti-Cd16a Antigen antibody
Anti-Fc-Gamma Riii-Alpha antibody
Anti-Fc-Gamma Riii antibody
Anti-Fc-Gamma Riiia antibody
Anti-Fcriii antibody
Anti-Fcriiia antibody
Anti-Fcr-10 antibody
Anti-Igg Fc Receptor Iii-2 antibody
Anti-Cd Antigen Cd16a antibody
Anti-FCGR3A antibody
Anti-CD16A antibody
Anti-FCG3 antibody
Anti-FCGR3 antibody
Anti-IGFR3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance