• Immunofluorescence analysis of HeLa cells using CCT4 Rabbit polyclonal antibody (STJ28631) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CCT4 antibody (STJ28631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CCT4 antibody (130-390) (STJ28631)

SKU:
STJ28631

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CCT4 (130-390) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCT4
Gene ID: 10575
Uniprot ID: TCPD_HUMAN
Immunogen Region: 130-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2).
Immunogen Sequence: PTIISESFQKALEKGIEILT DMSRPVELSDRETLLNSATT SLNSKVVSQYSSLLSPMSVN AVMKVIDPATATSVDLRDIK IVKKLGGTIDDCELVEGLVL TQKVSNSGITRVEKAKIGLI QFCLSAPKTDMDNQIVVSDY AQMDRVLREERAYILNLVKQ IKKTGCNVLLIQKSILRDAL SDLALHFLNKMKIMVIKDIE REDIEFICKTIGTKPVAHID QFTADMLGSAELAEEVNLN
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Protein Name T-Complex Protein 1 Subunit Delta
Tcp-1-Delta
Cct-Delta
Stimulator Of Tar Rna-Binding
Database Links Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-390450
Reactome: R-HSA-390471
Reactome: R-HSA-5620922
Reactome: R-HSA-6814122
Cellular Localisation Cytoplasm
Melanosome
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cilium Basal Body
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-T-Complex Protein 1 Subunit Delta antibody
Anti-Tcp-1-Delta antibody
Anti-Cct-Delta antibody
Anti-Stimulator Of Tar Rna-Binding antibody
Anti-CCT4 antibody
Anti-CCTD antibody
Anti-SRB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance