• Immunofluorescence analysis of U-2 OS cells using CCT3 antibody (STJ28630) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CCT3 antibody (STJ28630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CCT3 antibody (1-300) (STJ28630)

SKU:
STJ28630

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCT3
Gene ID: 7203
Uniprot ID: TCPG_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CCT3 (NP_005989.3).
Immunogen Sequence: MMGHRPVLVLSQNTKRESGR KVQSGNINAAKTIADIIRTC LGPKSMMKMLLDPMGGIVMT NDGNAILREIQVQHPAAKSM IEISRTQDEEVGDGTTSVII LAGEMLSVAEHFLEQQMHPT VVISAYRKALDDMISTLKKI SIPVDISDSDMMLNIINSSI TTKAISRWSSLACNIALDAV KMVQFEENGRKEIDIKKYAR VEKIPGGIIEDSCVLRGVMI NKDVTHPRMRRYIKNPRIV
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Protein Name T-Complex Protein 1 Subunit Gamma
Tcp-1-Gamma
Cct-Gamma
Chaperonin Containing T-Complex Polypeptide 1 Subunit 3
Htric5
Database Links Reactome: R-HSA-389957
Reactome: R-HSA-389960
Reactome: R-HSA-390450
Reactome: R-HSA-390471
Reactome: R-HSA-5620922
Reactome: R-HSA-6814122
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-T-Complex Protein 1 Subunit Gamma antibody
Anti-Tcp-1-Gamma antibody
Anti-Cct-Gamma antibody
Anti-Chaperonin Containing T-Complex Polypeptide 1 Subunit 3 antibody
Anti-Htric5 antibody
Anti-CCT3 antibody
Anti-CCTG antibody
Anti-TRIC5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance