Anti-CCR5 antibody (246-345) (STJ11103247)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CCR5 (246-345) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CCR5 |
| Gene ID | 1234 |
| Uniprot ID | CCR5_HUMAN |
| Immunogen Region | 246-345 |
| Immunogen Sequence | LFWAPYNIVLLLNTFQEFFG LNNCSSSNRLDQAMQVTETL GMTHCCINPIIYAFVGEKFR NYLLVFFQKHIAKRFCKCCS IFQQEAPERASSVYTRSTGE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 246-345 of human CCR5 (NP_000570.1). |
Additional Info
| Tissue Specificity | Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1a and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. |
| Post Translational Modifications | Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4. O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site even if Ser-7 may be also O-glycosylated. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen. Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry. Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. |
| Function | Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1. |
| Protein Name | C-C Chemokine Receptor Type 5C-C Ckr-5Cc-Ckr-5Ccr-5Ccr5Chemr13Hiv-1 Fusion CoreceptorCd Antigen Cd195 |
| Database Links | Reactome: R-HSA-173107Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6783783 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-C-C Chemokine Receptor Type 5 antibodyAnti-C-C Ckr-5 antibodyAnti-Cc-Ckr-5 antibodyAnti-Ccr-5 antibodyAnti-Ccr5 antibodyAnti-Chemr13 antibodyAnti-Hiv-1 Fusion Coreceptor antibodyAnti-Cd Antigen Cd195 antibodyAnti-CCR5 antibodyAnti-CMKBR5 antibody |
Information sourced from Uniprot.org