• Western blot analysis of extracts of various cell lines, using Cyclin E2 rabbit monoclonal antibody (STJ11102474) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using Cyclin E2 Rabbit monoclonal antibody (STJ11102474) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CCNE2 antibody (50-150) [S4MR] (STJ11102474)

SKU:
STJ11102474

£129.50 - £574.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNE2
Gene ID: 9134
Uniprot ID: CCNE2_HUMAN
Immunogen Region: 50-150
Specificity: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Cyclin E2 (O96020).
Immunogen Sequence: HQYEIRNCWPPVLSGGISPC IIIETPHKEIGTSDFSRFTN YRFKNLFINPSPLPDLSWGC SKEVWLNMLKKESRYVHDKH FEVLHSDLEPQMRSILLDWL L
Tissue Specificity highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. : low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.
Post Translational Modifications Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway. Lactylated at Lys-348. Delactylated by SIRT3.
Function Essential for the control of the cell cycle at the late G1 and early S phase.
Protein Name G1/S-Specific Cyclin-E2
Database Links Reactome: R-HSA-1538133
Reactome: R-HSA-187577
Reactome: R-HSA-2559586
Reactome: R-HSA-390471
Reactome: R-HSA-6804116
Reactome: R-HSA-69017
Reactome: R-HSA-69200
Reactome: R-HSA-69202
Reactome: R-HSA-69231
Reactome: R-HSA-69563
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Alternative Antibody Names Anti-G1/S-Specific Cyclin-E2 antibody
Anti-CCNE2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance