![Anti-CCND3 antibody [6H4] (STJ97979)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/120128/229673/STJ97979_2_ELISA__05913.1602854465.jpg?c=1 "Anti-CCND3 antibody [6H4] (STJ97979)")
![Anti-CCND3 antibody [6H4] (STJ97979)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/120128/229673/STJ97979_2_ELISA__05913.1602854465.jpg?c=1 "Anti-CCND3 antibody [6H4] (STJ97979)")
| Host: | Mouse |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-G1/S-specific cyclin-D3 is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 6H4 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CCND3 |
| Gene ID: | 896 |
| Uniprot ID: | CCND3_HUMAN |
| Specificity: | Cyclin D3 Monoclonal Antibody detects endogenous levels of Cyclin D3 protein. |
| Immunogen: | Purified recombinant fragment of human Cyclin D3 expressed in E. Coli. |
| Function | Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4. |
| Protein Name | G1/S-Specific Cyclin-D3 |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-5687128Reactome: R-HSA-69231Reactome: R-HSA-8934593Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-G1/S-Specific Cyclin-D3 antibodyAnti-CCND3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and Cyclin D3 knockout (KO) HeLa cells, using [KO Validated] Cyclin D3 Rabbit polyclonal antibody (STJ22949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99291/384122/STJ22949_1__40172.1713152776.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Cyclin D3 knockout (KO) HeLa cells, using [KO Validated] Cyclin D3 Rabbit polyclonal antibody (STJ22949) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
