| Host: |
Mouse |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat/Cow/Dog/Pig |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Chromobox protein homolog 5 is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Purification: |
Affinity purification |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000-1:2000IF 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CBX5 |
| Gene ID: |
23468 |
| Uniprot ID: |
CBX5_HUMAN |
| Specificity: |
HP1 Alpha Monoclonal Antibody detects endogenous levels of HP1 Alpha protein. |
| Immunogen: |
Purified recombinant human HP1 Alpha protein fragments expressed in E.coli. |
| Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Protein Name | Chromobox Protein Homolog 5Antigen P25Heterochromatin Protein 1 Homolog AlphaHp1 Alpha |
| Database Links | Reactome: R-HSA-4551638Reactome: R-HSA-8953750Reactome: R-HSA-983231 |
| Cellular Localisation | NucleusChromosomeCentromereColocalizes With Hnrnpu In The NucleusComponent Of Centromeric And Pericentromeric HeterochromatinAssociates With Chromosomes During MitosisAssociates Specifically With Chromatin During Metaphase And AnaphaseLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 5 antibodyAnti-Antigen P25 antibodyAnti-Heterochromatin Protein 1 Homolog Alpha antibodyAnti-Hp1 Alpha antibodyAnti-CBX5 antibodyAnti-HP1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of U2OS cells using [KO Validated] HP1 alpha/CBX5 Rabbit polyclonal antibody (STJ114451) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/93564/374717/STJ114451_1__25554.1713141286.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] HP1 alpha/CBX5 Rabbit polyclonal antibody (STJ114451) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")