| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC/IF/FC |
| Reactivity: | Human/Mouse/Rat/Cow/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CBX3/HP1 Gamma (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx. 20kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CBX3 |
| Gene ID: | 11335 |
| Uniprot ID: | CBX3_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_009207.2 |
| Specificity: | No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2 |
| Immunogen Sequence: | EAFLNSQKAGKEKD |
| Function | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs). |
| Protein Name | Chromobox Protein Homolog 3 Hech Heterochromatin Protein 1 Homolog Gamma Hp1 Gamma Modifier 2 Protein |
| Database Links | Reactome: R-HSA-427389 Reactome: R-HSA-73772 Reactome: R-HSA-8953750 |
| Cellular Localisation | Nucleus Associates With Euchromatin And Is Largely Excluded From Constitutive Heterochromatin May Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential) |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 3 antibody Anti-Hech antibody Anti-Heterochromatin Protein 1 Homolog Gamma antibody Anti-Hp1 Gamma antibody Anti-Modifier 2 Protein antibody Anti-CBX3 antibody |
Information sourced from Uniprot.org