• STJ70791 (0. 03µg/ml) staining of A431 (A) , HeLa (B) , HepG2 (C) , Jurkat (D) and K562 (E) nuclear cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70791 (0. 1µg/ml) staining of NIH3T3 nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70791 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70791 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70791 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-CBX3/HP1 Gamma antibody (Internal) (STJ70791)

SKU:
STJ70791-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Cow/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CBX3/HP1 Gamma (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1.0 µg/ml
IHC-2-3µg/ml
IF-Strong expression of the protein seen in HeLa and U2OS cells. 10µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CBX3
Gene ID: 11335
Uniprot ID: CBX3_HUMAN
Immunogen Region: Internal
Accession Number: NP_009207.2
Specificity: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2
Immunogen Sequence: EAFLNSQKAGKEKD
Function Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs).
Protein Name Chromobox Protein Homolog 3
Hech
Heterochromatin Protein 1 Homolog Gamma
Hp1 Gamma
Modifier 2 Protein
Database Links Reactome: R-HSA-427389
Reactome: R-HSA-73772
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Associates With Euchromatin And Is Largely Excluded From Constitutive Heterochromatin
May Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential)
Alternative Antibody Names Anti-Chromobox Protein Homolog 3 antibody
Anti-Hech antibody
Anti-Heterochromatin Protein 1 Homolog Gamma antibody
Anti-Hp1 Gamma antibody
Anti-Modifier 2 Protein antibody
Anti-CBX3 antibody

Information sourced from Uniprot.org

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