• STJ71980 (0. 1µg/ml) staining of Human Cerebellum (A) and Colorectal cancer (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71980 (0. 1µg/ml) staining of CaCo-2 (A) and (1ug/ml) NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71980 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Calnexin antibody (Internal) (STJ71980)

SKU:
STJ71980-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Calnexin (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-5µg/ml
IF-Strong expression of the protein seen in the endoplasmic reticulum (ER) of NIH3T3 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CANX
Gene ID: 821
Uniprot ID: CALX_HUMAN
Immunogen Region: Internal
Accession Number: NP_001737.1
Specificity: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).
Immunogen Sequence: SKTPELNLDQFHDKT
Function Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
Protein Name Calnexin
Ip90
Major Histocompatibility Complex Class I Antigen-Binding Protein P88
P90
Database Links Reactome: R-HSA-168316
Reactome: R-HSA-2132295
Reactome: R-HSA-8984722
Reactome: R-HSA-901042
Reactome: R-HSA-9020956
Reactome: R-HSA-9683686
Reactome: R-HSA-9694548
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
The Palmitoylated Form Preferentially Localizes To The Perinuclear Rough Er
Alternative Antibody Names Anti-Calnexin antibody
Anti-Ip90 antibody
Anti-Major Histocompatibility Complex Class I Antigen-Binding Protein P88 antibody
Anti-P90 antibody
Anti-CANX antibody

Information sourced from Uniprot.org

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