• Immunofluorescence analysis of mouse testis cells using Calmegin Rabbit monoclonal antibody (STJ11103070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat testis cells using Calmegin Rabbit monoclonal antibody (STJ11103070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using Calmegin Rabbit monoclonal antibody (STJ11103070) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using Calmegin Rabbit monoclonal antibody (STJ11103070) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of PC-3 cells, using Calmegin Rabbit monoclonal antibody (STJ11103070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Calmegin Rabbit monoclonal antibody (STJ11103070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Calmegin antibody (180-350) [S0MR] (STJ11103070)

SKU:
STJ11103070

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Calmegin (180-350) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLGN
Gene ID: 1047
Uniprot ID: CLGN_HUMAN
Immunogen Region: 180-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-350 of human Calmegin (O14967).
Immunogen Sequence: FGPDKCGEDYKLHFIFRHKH PKTGVFEEKHAKPPDVDLKK FFTDRKTHLYTLVMNPDDTF EVLVDQTVVNKGSLLEDVVP PIKPPKEIEDPNDKKPEEWD ERAKIPDPSAVKPEDWDESE PAQIEDSSVVKPAGWLDDEP KFIPDPNAEKPDDWNEDTDG EWEAPQILNPA
Tissue Specificity Detected in testis (at protein level). Detected in testis.
Function Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.
Protein Name Calmegin
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Calmegin antibody
Anti-CLGN antibody

Information sourced from Uniprot.org

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