• Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using CAL Rabbit polyclonal antibody (STJ11104026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CAL antibody (1-80 aa) (STJ11104026)

SKU:
STJ11104026

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Host: Rabbit
Applications: WB/ELISA
Reactivity: O.sativa
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-80 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of Oryza sativa CAL. (Q6K209).
Immunogen Sequence: MAPSRRMVASAFLLLAILVA TEMGTTKVAEARHCLSQSHR FKGMCVSSNNCANVCRTESF PDGECKSHGLERKCFCKKVC
Background Plant defensin-like protein involved in accumulation of cadmium (Cd) in rice leaves. Mediates Cd efflux from cytosol into extracellular spaces via chelation. This drives Cd secretion from xylem parenchyma cells into the xylem vessels, hence lowering Cd levels in cytosol meanwhile promoting Cd translocation from roots to shoots.

Information sourced from Uniprot.org

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