| Host: |
Rabbit |
| Applications: |
WB/IHC-P/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CAD |
| Gene ID: |
790 |
| Uniprot ID: |
PYR1_HUMAN |
| Immunogen Region: |
1650-1900 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2). |
| Immunogen Sequence: |
LERLGPGKGEVRPELGSRQD VEALWENMAVIDCFASDHAP HTLEEKCGSRPPPGFPGLET MLPLLLTAVSEGRLSLDDLL QRLHHNPRRIFHLPPQEDTY VEVDLEHEWTIPSHMPFSKA HWTPFEGQKVKGTVRRVVLR GEVAYIDGQVLVPPGYGQDV RKWPQGAVPQLPPSAPATSE MTTTPERPRRGIPGLPDGRF HLPPRIHRASDPGLPAEEPK EKSSRKVAEPELMGTPDGT |
| Post Translational Modifications | Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP. |
| Function | Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase.EC 6.3.5.5), aspartate transcarbamylase (ATCase.EC 2.1.3.2) and dihydroorotase (DHOase.EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate. |
| Protein Name | Multifunctional Protein CadCarbamoyl Phosphate Synthetase 2-Aspartate Transcarbamylase-Dihydroorotase Includes - Glutamine-Dependent Carbamoyl Phosphate Synthase - Glutamine AmidotransferaseGataseGlnase - Ammonium-Dependent Carbamoyl Phosphate SynthaseCpsCpsase - Aspartate Carbamoyltransferase - Dihydroorotase |
| Database Links | Reactome: R-HSA-500753 |
| Cellular Localisation | CytoplasmNucleusCytosolic And Unphosphorylated In Resting CellsTranslocates To The Nucleus In Response To Egf StimulationNuclear Import Promotes Optimal Cell Growth |
| Alternative Antibody Names | Anti-Multifunctional Protein Cad antibodyAnti-Carbamoyl Phosphate Synthetase 2-Aspartate Transcarbamylase-Dihydroorotase Includes - Glutamine-Dependent Carbamoyl Phosphate Synthase - Glutamine Amidotransferase antibodyAnti-Gatase antibodyAnti-Glnase - Ammonium-Dependent Carbamoyl Phosphate Synthase antibodyAnti-Cps antibodyAnti-Cpsase - Aspartate Carbamoyltransferase - Dihydroorotase antibodyAnti-CAD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
