• Western blot analysis of extracts of various cell lines, using CAD antibody (STJ110642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U-2 OS cells using CAD Rabbit polyclonal antibody (STJ110642) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human tonsil using CAD antibody (STJ110642) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of NIH-3T3 cells using CAD Rabbit polyclonal antibody (STJ110642) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat testis using CAD antibody (STJ110642) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of CAD in paraffin-embedded mouse spleen using CAD Rabbit polyclonal antibody (STJ110642) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using CAD antibody (STJ110642) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of CAD in paraffin-embedded rat testis using CAD Rabbit polyclonal antibody (STJ110642) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using CAD antibody (STJ110642) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CAD in paraffin-embedded human tonsil using CAD Rabbit polyclonal antibody (STJ110642) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using CAD antibody (STJ110642) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CAD Rabbit polyclonal antibody (STJ110642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CAD antibody (1650-1900) (STJ110642)

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STJ110642

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CAD (1650-1900) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CAD
Gene ID: 790
Uniprot ID: PYR1_HUMAN
Immunogen Region: 1650-1900
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).
Immunogen Sequence: LERLGPGKGEVRPELGSRQD VEALWENMAVIDCFASDHAP HTLEEKCGSRPPPGFPGLET MLPLLLTAVSEGRLSLDDLL QRLHHNPRRIFHLPPQEDTY VEVDLEHEWTIPSHMPFSKA HWTPFEGQKVKGTVRRVVLR GEVAYIDGQVLVPPGYGQDV RKWPQGAVPQLPPSAPATSE MTTTPERPRRGIPGLPDGRF HLPPRIHRASDPGLPAEEPK EKSSRKVAEPELMGTPDGT
Post Translational Modifications Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP.
Function This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Protein Name Cad Protein Includes - Glutamine-Dependent Carbamoyl-Phosphate Synthase - Aspartate Carbamoyltransferase - Dihydroorotase
Database Links Reactome: R-HSA-500753
Cellular Localisation Cytoplasm
Nucleus
Cytosolic And Unphosphorylated In Resting Cells
Translocates To The Nucleus In Response To Egf Stimulation
Nuclear Import Promotes Optimal Cell Growth
Alternative Antibody Names Anti-Cad Protein Includes - Glutamine-Dependent Carbamoyl-Phosphate Synthase - Aspartate Carbamoyltransferase - Dihydroorotase antibody
Anti-CAD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance