| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CA5B |
| Gene ID: | 11238 |
| Uniprot ID: | CAH5B_HUMAN |
| Immunogen Region: | 1-317 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human CA5B (NP_009151.1). |
| Immunogen Sequence: | MVVMNSLRVILQASPGKLLW RKFQIPRFMPARPCSLYTCT YKTRNRALHPLWESVDLVPG GDRQSPINIRWRDSVYDPGL KPLTISYDPATCLHVWNNGY SFLVEFEDSTDKSVIKGGPL EHNYRLKQFHFHWGAIDAWG SEHTVDSKCFPAELHLVHWN AVRFENFEDAALEENGLAVI GVFLKLGKHHKELQKLVDTL PSIKHKDALVEFGSFDPSCL MPTCPDYWTYSGSLTTPPL |
| Tissue Specificity | Strongest expression in heart, pancreas, kidney, placenta, lung, and skeletal muscle. Not expressed in liver. |
| Function | Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3. |
| Protein Name | Carbonic Anhydrase 5b - MitochondrialCarbonate Dehydratase VbCarbonic Anhydrase VbCa-Vb |
| Database Links | Reactome: R-HSA-1475029 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Carbonic Anhydrase 5b - Mitochondrial antibodyAnti-Carbonate Dehydratase Vb antibodyAnti-Carbonic Anhydrase Vb antibodyAnti-Ca-Vb antibodyAnti-CA5B antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and HMG20B knockout (KO) 293T cells, using [KO Validated] HMG20B Rabbit polyclonal antibody (STJ11100827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89763/358238/STJ11100827_1__18600.1713122571.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HMG20B knockout (KO) 293T cells, using [KO Validated] HMG20B Rabbit polyclonal antibody (STJ11100827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
