Anti-C9orf114 antibody (163-340) (STJ11100414)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal C9orf114 (163-340) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPOUT1 |
| Gene ID | 51490 |
| Uniprot ID | SPOUT_HUMAN |
| Immunogen Region | 163-340 |
| Immunogen Sequence | HQDLQFAGLLNPLDSPHHMR QDEESEFREGIVVDRPTRPG HGSFVNCGMKKEVKIDKNLE PGLRVTVRLNQQQHPDCKTY HGKVVSSQDPRTKAGLYWGY TVRLASCLSAVFAEAPFQDG YDLTIGTSERGSDVASAQLP NFRHALVVFGGLQGLEAGAD ADPNLEVAEPSVLFDLYV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 163-340 of human C9orf114 (NP_057474.2). |
Additional Info
| Function | S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level. |
| Protein Name | 28s RrnaUridine-N(3-MethyltransferaseCentromere Protein 32Cenp-32Kinetochore-Associated ProteinMethyltransferase C9orf114Ribosomal Rna Methyltransferase Spout1Spout Domain-Containing Methyltransferase 1 |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonSpindleChromosomeCentromereKinetochoreMicrotubule Organizing CenterCentrosomeAssociated With The Outer Kinetochore |
| Alternative Antibody Names | Anti-28s Rrna antibodyAnti-Uridine-N(3-Methyltransferase antibodyAnti-Centromere Protein 32 antibodyAnti-Cenp-32 antibodyAnti-Kinetochore-Associated Protein antibodyAnti-Methyltransferase C9orf114 antibodyAnti-Ribosomal Rna Methyltransferase Spout1 antibodyAnti-Spout Domain-Containing Methyltransferase 1 antibodyAnti-SPOUT1 antibodyAnti-C9orf114 antibody |
Information sourced from Uniprot.org