• Immunohistochemistry analysis of C9orf114 in paraffin-embedded Rat kidney using C9orf114 Rabbit polyclonal antibody (STJ11100414) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of C9orf114 in paraffin-embedded Human liver cancer using C9orf114 Rabbit polyclonal antibody (STJ11100414) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using C9orf114 Rabbit polyclonal antibody (STJ11100414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-C9orf114 antibody (163-340) (STJ11100414)

SKU:
STJ11100414

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C9orf114 (163-340) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPOUT1
Gene ID: 51490
Uniprot ID: CI114_HUMAN
Immunogen Region: 163-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 163-340 of human C9orf114 (NP_057474.2).
Immunogen Sequence: HQDLQFAGLLNPLDSPHHMR QDEESEFREGIVVDRPTRPG HGSFVNCGMKKEVKIDKNLE PGLRVTVRLNQQQHPDCKTY HGKVVSSQDPRTKAGLYWGY TVRLASCLSAVFAEAPFQDG YDLTIGTSERGSDVASAQLP NFRHALVVFGGLQGLEAGAD ADPNLEVAEPSVLFDLYV
Function Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level.
Protein Name Putative Methyltransferase C9orf114
Centromere Protein 32
Cenp-32
Kinetochore-Associated Protein
Spout Domain-Containing Methyltransferase 1
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Chromosome
Centromere
Kinetochore
Microtubule Organizing Center
Centrosome
Associated With The Outer Kinetochore
Alternative Antibody Names Anti-Putative Methyltransferase C9orf114 antibody
Anti-Centromere Protein 32 antibody
Anti-Cenp-32 antibody
Anti-Kinetochore-Associated Protein antibody
Anti-Spout Domain-Containing Methyltransferase 1 antibody
Anti-SPOUT1 antibody
Anti-C9orf114 antibody

Information sourced from Uniprot.org

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