• Western blot analysis of extracts of various cell lines, using C19orf10 antibody (STJ22849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using C19orf10 rabbit polyclonal antibody (STJ22849) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using C19orf10 rabbit polyclonal antibody (STJ22849) at dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-C19orf10 antibody (1-173) (STJ22849)

SKU:
STJ22849

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C19orf10 (1-173) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYDGF
Gene ID: 56005
Uniprot ID: MYDGF_HUMAN
Immunogen Region: 1-173
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human C19orf10 (NP_061980.1).
Immunogen Sequence: MAAPSGGWNGVGASLWAALL LGAVALRPAEAVSEPTTVAF DVRPGGVVHSFSHNVGPGDK YTCMFTYASQGGTNEQWQMS LGTSEDHQHFTCTIWRPQGK SYLYFTQFKAEVRGAEIEYA MAYSKAAFERESDVPLKTEE FEVTKTAVAHRPGAFKAELS KLVIVAKASRTEL
Tissue Specificity Expressed in eosinophils (at protein level). Expressed in bone marrow cells. Expressed in synovial tissue. Found in synovial fluid of patients with arthropaties.
Function Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway. Involved in endothelial cell proliferation and angiogenesis.
Protein Name Myeloid-Derived Growth Factor
Mydgf
Database Links Reactome: R-HSA-381038
Cellular Localisation Secreted
Endoplasmic Reticulum-Golgi Intermediate Compartment
Endoplasmic Reticulum
Golgi Apparatus
The C-Terminal Rtel Motif May Provide Retention In The Endoplasmic Reticulum
Alternative Antibody Names Anti-Myeloid-Derived Growth Factor antibody
Anti-Mydgf antibody
Anti-MYDGF antibody
Anti-C19orf10 antibody

Information sourced from Uniprot.org

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