• Immunohistochemistry analysis of BYSL in paraffin-embedded human breast cancer using BYSL Rabbit polyclonal antibody (STJ113897) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BYSL in paraffin-embedded human colon carcinoma using BYSL Rabbit polyclonal antibody (STJ113897) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BYSL in paraffin-embedded rat ovary using BYSL Rabbit polyclonal antibody (STJ113897) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BYSL Rabbit polyclonal antibody (STJ113897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BYSL antibody (120-280) (STJ113897)

SKU:
STJ113897

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BYSL (120-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BYSL
Gene ID: 705
Uniprot ID: BYST_HUMAN
Immunogen Region: 120-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human BYSL (NP_004044.3).
Immunogen Sequence: HAEVVVDPEDERAIEMFMNK NPPARRTLADIIMEKLTEKQ TEVETVMSEVSGFPMPQLDP RVLEVYRGVREVLSKYRSGK LPKAFKIIPALSNWEQILYV TEPEAWTAAAMYQATRIFAS NLKERMAQRFYNLVLLPRVR DDVAEYKRLNFHLYMALKKA L
Tissue Specificity Found in the placenta from the sixth week of pregnancy. Was localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the level decreased and then disappeared from placental villi.
Function Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. May be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos.
Protein Name Bystin
Database Links Reactome: R-HSA-6791226
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Associated With 40s Ribosomal Subunits
Alternative Antibody Names Anti-Bystin antibody
Anti-BYSL antibody
Anti-ENP1 antibody

Information sourced from Uniprot.org

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