| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BUB3 (1-328) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BUB3 |
| Gene ID: |
9184 |
| Uniprot ID: |
BUB3_HUMAN |
| Immunogen Region: |
1-328 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1). |
| Immunogen Sequence: |
MTGSNEFKLNQPPEDGISSV KFSPNTSQFLLVSSWDTSVR LYDVPANSMRLKYQHTGAVL DCAFYDPTHAWSGGLDHQLK MHDLNTDQENLVGTHDAPIR CVEYCPEVNVMVTGSWDQTV KLWDPRTPCNAGTFSQPEKV YTLSVSGDRLIVGTAGRRVL VWDLRNMGYVQQRRESSLKY QTRCIRAFPNKQGYVLSSIE GRVAVEYLDPSPEVQKKKYA FKCHRLKENNIEQIYPVNA |
| Post Translational Modifications | Poly-ADP-ribosylated by PARP1. |
| Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
| Protein Name | Mitotic Checkpoint Protein Bub3 |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174184Reactome: R-HSA-176409Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreStarts To Localize At Kinetochores In Prometaphase I (Pro-Mi) Stage And Maintains The Localization Until The Metaphase I-Anaphase I (Mi-Ai) Transition |
| Alternative Antibody Names | Anti-Mitotic Checkpoint Protein Bub3 antibodyAnti-BUB3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
