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Anti-BRPF1 antibody (1-200) (STJ11102828)

Immunofluorescence analysis of U-2 OS cells using BRPF1 Rabbit polyclonal antibody (STJ11102828) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using BRPF1 Rabbit polyclonal antibody (STJ11102828) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using BRPF1 Rabbit polyclonal antibody (STJ11102828) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of lysates from Mouse testis, using BRPF1 Rabbit polyclonal antibody (STJ11102828) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BRPF1 antibody (1-200) (STJ11102828)

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STJ11102828

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-BRPF1 (1-200) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	BRPF1
Gene ID:	7862
Uniprot ID:	BRPF1_HUMAN
Immunogen Region:	1-200
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BRPF1 (NP_001003694).
Immunogen Sequence:	MGVDFDVKTFCHNLRATKPP YECPVETCRKVYKSYSGIEY HLYHYDHDNPPPPQQTPLRK HKKKGRQSRPANKQSPSPSE VSQSPGREVMSYAQAQRMVE VDLHGRVHRISIFDNLDVVS EDEEAPEEAPENGSNKENTE TPAATPKSGKHKNKEKRKDS NHHHHHNVSASTTPKLPEVV YRELEQDTPDAPPRPTSYYR

Tissue Specificity	High levels in testis.
Post Translational Modifications	Acetylated by KAT6A.
Function	Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity. Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac). Some HAT complexes preferentially mediate histone H3 'Lys-23' (H3K23ac) acetylation. Positively regulates the transcription of RUNX1 and RUNX2.
Protein Name	Peregrin Bromodomain And Phd Finger-Containing Protein 1 Protein Br140
Database Links	Reactome: R-HSA-3214847 Reactome: R-HSA-6804758
Cellular Localisation	Nucleus Chromosome Cytoplasm Localization To The Nucleus Depends On Kat6a Ing5 And Meaf6 Localizes To Transcription Start Sites
Alternative Antibody Names	Anti-Peregrin antibody Anti-Bromodomain And Phd Finger-Containing Protein 1 antibody Anti-Protein Br140 antibody Anti-BRPF1 antibody Anti-BR140 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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