| Short Description : | Rabbit polyclonal BRMS1 (90-210) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:100IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BRMS1 |
| Gene ID: | 25855 |
| Uniprot ID: | BRMS1_HUMAN |
| Immunogen Region: | 90-210 |
| Immunogen Sequence: | LEEVGAERAPEYTEPLGGLQ RSLKIRIQVAGIYKGFCLDV IRNKYECELQGAKQHLESEK LLLYDTLQGELQERIQRLEE DRQSLDLSSEWWDDKLHARG SSRSWDSLPPSKRKKAPLVS G |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1). |
| Tissue Specificity | Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies. |
| Post Translational Modifications | Ubiquitinated by a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing SPOP, leading to proteasomal degradation. |
| Function | Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. |
| Protein Name | Breast Cancer Metastasis-Suppressor 1 |
| Database Links | Reactome: R-HSA-3214815Reactome: R-HSA-9679191 |
| Cellular Localisation | NucleusCytoplasmPredominantly Nuclear |
| Alternative Antibody Names | Anti-Breast Cancer Metastasis-Suppressor 1 antibodyAnti-BRMS1 antibody |
Information sourced from Uniprot.org
