| Host: |
Goat |
| Applications: |
Pep-ELISA/IF |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-BRM (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IF-Strong expression of the protein seen in the Nucleus and some staining in the Cytoplasm of HeLa and U251 cells. 10µg/mlELISA-antibody detection limit dilution 1:1000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
SMARCA2 |
| Gene ID: |
6595 |
| Uniprot ID: |
SMCA2_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_003061.3; NP_620614.2 |
| Specificity: |
This antibody is expected to recognise both reported isoforms (NP_003061.3; NP_620614.2) |
| Immunogen Sequence: |
PSNSQLEIEGNSS |
| Function | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. |
| Protein Name | Probable Global Transcription Activator Snf2l2Atp-Dependent Helicase Smarca2Brg1-Associated Factor 190bBaf190bProtein Brahma HomologHbrmSnf2-AlphaSwi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2 |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8939243 |
| Cellular Localisation | NucleusLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Probable Global Transcription Activator Snf2l2 antibodyAnti-Atp-Dependent Helicase Smarca2 antibodyAnti-Brg1-Associated Factor 190b antibodyAnti-Baf190b antibodyAnti-Protein Brahma Homolog antibodyAnti-Hbrm antibodyAnti-Snf2-Alpha antibodyAnti-Swi/Snf-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily A Member 2 antibodyAnti-SMARCA2 antibodyAnti-BAF190B antibodyAnti-BRM antibodyAnti-SNF2A antibodyAnti-SNF2L2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
