• Immunofluorescence analysis of U-2 OS cells using BRK1 Rabbit polyclonal antibody (STJ119333) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of BRK1 in paraffin-embedded human stomach using BRK1 Rabbit polyclonal antibody (STJ119333) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BRK1 in paraffin-embedded human breast cancer using BRK1 Rabbit polyclonal antibody (STJ119333) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-BRK1 antibody (1-75) (STJ119333)

SKU:
STJ119333

£112.50 - £522.50
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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRK1 (1-75) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRK1
Gene ID: 55845
Uniprot ID: BRK1_HUMAN
Immunogen Region: 1-75
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human BRK1 (NP_060932.2).
Immunogen Sequence: MAGQEDPVQREIHQDWANRE YIEIITSSIKKIADFLNSFD MSCRSRLATLNEKLTALERR IEYIEARVTKGETLT
Function Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
Protein Name Protein Brick1
Brk1
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-4420097
Reactome: R-HSA-5663213
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Protein Brick1 antibody
Anti-Brk1 antibody
Anti-BRK1 antibody
Anti-C3orf10 antibody
Anti-HSPC300 antibody
Anti-MDS027 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance