• Immunohistochemistry analysis of Brdu. Sample: mouse jejunum, 4% PFA 12-24h. Antigen retrieval:2M HCL in pure water, 37C, 30min Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of Brdu. Sample: mouse jejunum, 4% PFA 12-24h. Antigen retrieval:2M HCL in pure water, 37C, 30min Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • IF analysis of Brdu. Sample: mouse neonatal mouse NA s brain, 4% PFA 12-24h. Antigen retrieval: TE buffer, 98C, 20min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:500, 4C overnight. Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG, 1:200 RT 1h.

Anti-BrdU antibody [SM5171] (STJA0005171)

SKU:
STJA0005171

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Host: Mouse
Applications: IHC/IF
Reactivity: All
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-BrdU is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM5171
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: IHC/IF (All species) 1:100-1:500/1:100-1:500
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse BrdU
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance